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                  Titre : Philosophical transactions of the Royal society of London. Series B, Biological sciences

                  Titre : Philosophical transactions of the Royal society of London. B

                  Auteur : Royal society (GB)

                  Éditeur : printed by Harrison and sons (London)

                  Éditeur : The Royal society (London)

                  Date d'édition : 1888-1990

                  Type : texte,publication en série imprimée

                  Langue : Anglais

                  Format : application/pdf

                  Droits : domaine public

                  Identifiant : ark:/12148/cb34422226r/date

                  Identifiant : ISSN 00804622

                  Source : Bibliothèque nationale de France

                  Relation : http://catalogue.bnf.fr/ark:/12148/cb34422226r

                  Description : Variante(s) de titre : Philosophical transactions of the Royal society of London

                  Description : Variante(s) de titre : Philosophical transactions of the Royal society of London

                  Description : Périodicité : Irrégulier

                  Description : Etat de collection : 1888 (vol. 178)-1939

                  Provenance : bnf.fr

                  Date de mise en ligne : 15/10/2007

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                  First issue for the year 1908 Previous issue 1908 (VOL199). Next issue Last issue for the year 1908
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                  Title : Philosophical transactions of the Royal society of London. Series B, Biological sciences

                  Author : Royal society (GB)

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                  DEVELOPMENT OF LARVAL NEPHRIDIA. 225
                  If not fed. after the third dav thev gradually dimmish in siz»

                  ordinary sea-water. If not fed, after thé third day they gradually dimfoish in siz»
                  and- finally break up at the'end of a few weeks, having made no further progress in
                  devèlopment. .S~M~SS~
                  As the adult worms are by po means very abundant at Naples, and thé number of
                  eggs obtained from a single worm is never very great, I experienced considérable
                  difliculty in obtaining sufficiènt material. On this account I adopted the môst
                  economical method of studying the material I obtained. 1 found the combiiled
                  celloidin-parafhn method of sectioning very useful, as by the use of this raethod very
                  little material is lost in the process ot embedding, and it is possible to section the
                  minutest larvée one at a time if necessary. It also has this advantage, that very
                  small sections can be traced in a rapid manner consecutively on the slide, by means
                  of the celloidin. On this account 1 did not adopt thé usual plan of dissolvïng it out,
                  but allowed it to remain on the slide as a means of readily finding the sections.
                  Material was fixed either in Flemming's strong solution, or Hermann's. For staining,
                  hasmacalcium and otlier hsematoxylin mixtures are most useful. For larvée to be
                  studied whole, dilute paracarmine, followed by slightly acid alcohol, gives very
                  satisfactory results.,

                  While the larva of Polygordius is found in thé tow abundantly during onlythe
                  months of February, March, and April, it is possible to rear the larva from the egg
                  throughout ail the spring and summer months. After the middle of September I had
                  some difticulty in obtaining the adults, so I have been unable to détermine whether
                  the sexual products are developed all the year round. The normal discharge of thèse
                  products seems to be restricted to the spring and, possibly, the summer months.
                  The free-swimming larvœ are found only during the spring and summer months
                  along the eastern coast of North America and. in the North Sea, while in the
                  Mediterranean they are found in February, March, and April, as already mentioned.
                  During the month of May, 1900, 1 obtained a number of Polygordius larvae from the
                  "tow" at Woods Holl, Massachusetts, which 1 had the opportunity of comparing
                  with the larvae found at*Naples; from these they can hardly be distinguished, and in
                  all essential featm-es, as I have already mentioned, they are the same.
                  I have considered it best in the foregoing work not to mention the various stages
                  by age, as development is affected by température to such an extent that there
                  is some uncertainty in designating stages by this^fuean's. The' progress made in
                  development in warm weather is twice as great as in the cold months of the early
                  spring. It is possible that under natural conditions developmeut may be still more
                  rapid for this reason time is a somewhat incertain manner of designating them.
                  Cowlks(3) states that he found the larva of P. appendieulatus go through metamor-
                  phosis in the course of a night under favourable conditions. He says, It surprised
                  me tofind that fully-developed swimming larvée, takén at night; and measuring slightly
                  less than 1 millim. in length, had metàmorphosed by the ilext morning. They were
                  at this time 2 millims. in length, showing an increase of 1 millim. in length (p. 125).

                  7 Q
                  VOL. OXCIX. B. 2 G


                  Source: gallica.bnf.fr / Bibliothèque nationale de France

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